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Journal Article

Isolation of functional extensor and flexor protoplasts from Phaseolus coccineus L. pulvini: potassium induced swelling

F. Erath, W.A. Ruge, W.-E. Mayer and R. Hampp
Planta
Vol. 173, No. 4 (1988), pp. 447-452
Published by: Springer
Stable URL: http://www.jstor.org/stable/23379149
Page Count: 6

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Topics: Protoplasts, Flexors, Pulvini, Plants, Guard cells, Plant cells, Mesophyll, Swelling, Oxygen, Potassium
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Isolation of functional extensor and flexor protoplasts from Phaseolus coccineus L. pulvini: potassium induced swelling
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Abstract

Methods are described for the isolation of functional protoplasts from secondary pulvinus tissue (flexor and extensor) and from leaf mesophyll tissue of primary leaves of Phaseolus coccineus L. Integrity of the protoplasts was shown by vital staining and their ability to evolve oxygen in the light. Extensor-cell protoplasts increased their volume for up to 60% upon addition of 10 mM KCl. This K+-induced swelling was accompanied by increased rates of proton extrusion.

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