Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

Isolation of functional extensor and flexor protoplasts from Phaseolus coccineus L. pulvini: potassium induced swelling

F. Erath, W.A. Ruge, W.-E. Mayer and R. Hampp
Planta
Vol. 173, No. 4 (1988), pp. 447-452
Published by: Springer
Stable URL: http://www.jstor.org/stable/23379149
Page Count: 6
  • More info
  • Cite this Item
Isolation of functional extensor and flexor protoplasts from Phaseolus coccineus L. pulvini: potassium induced swelling
Preview not available

Abstract

Methods are described for the isolation of functional protoplasts from secondary pulvinus tissue (flexor and extensor) and from leaf mesophyll tissue of primary leaves of Phaseolus coccineus L. Integrity of the protoplasts was shown by vital staining and their ability to evolve oxygen in the light. Extensor-cell protoplasts increased their volume for up to 60% upon addition of 10 mM KCl. This K+-induced swelling was accompanied by increased rates of proton extrusion.

Page Thumbnails

  • Thumbnail: Page 
[447]
    [447]
  • Thumbnail: Page 
448
    448
  • Thumbnail: Page 
449
    449
  • Thumbnail: Page 
450
    450
  • Thumbnail: Page 
451
    451
  • Thumbnail: Page 
452
    452