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The detection of fucose residues in plant nuclear envelopes
Peter J. Gruber, Kathleen A. Sweeney and Sue Ellen Frederick
Vol. 174, No. 3 (1988), pp. 298-304
Published by: Springer
Stable URL: http://www.jstor.org/stable/23379261
Page Count: 7
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Protoplasts from suspension-cultured Daucus carota L. cells, when fixed and incubated with fluorescein conjugates of the fucosyl-specific lectin Ulex europaeus agglutinin I, exhibited the following pattern of labeling: plasma membranes were not marked, but striking halos of fluorescence appeared around the periphery of all nuclei. Identical observations were made with protoplasts from Vicia faba L. leaves, Pisum sativum L. epicotyls, Zea mays L. roots and Glycine max L. cell suspensions, as well as with nuclei in cell-free preparations from the same sources. These results indicate that in a broad spectrum of angiosperm cells, fucose residues are associated with the nuclear envelope. The relationship of this finding in plant cells to recent discoveries regarding nuclear glycoconjugates in animal cells remains to be explored.
Planta © 1988 Springer