Access

You are not currently logged in.

Access JSTOR through your library or other institution:

login

Log in through your institution.

Journal Article

Immunocytochemical localization of myrosinase in Brassica napus L.

O.P. Thangstad, T.-H. Iversen, G. Slupphaug and A. Bones
Planta
Vol. 180, No. 2 (1990), pp. 245-248
Published by: Springer
Stable URL: http://www.jstor.org/stable/23380105
Page Count: 4

You can always find the topics here!

Topics: Brasses, Cotyledons, Fluorescence, Antibodies, Glucosinolates, Enzymes, Rape, Mustards, Geodetic position, Oilseeds
Were these topics helpful?
See somethings inaccurate? Let us know!

Select the topics that are inaccurate.

Cancel
  • More info
  • Add to My Lists
  • Cite this Item
Immunocytochemical localization of myrosinase in Brassica napus L.
Preview not available

Abstract

The cytological and intracellular localization of myrosinase (EC 3.2.3.1) has been studied by immunochemical techniques using paraffin-embedded sections of radicles and cotyledons from seeds of Brassica napus L. cv. Niklas. For immunolabelling, sections were sequentially incubated with a monoclonal anti-myrosinase antiboyd and with peroxidase- and fluorescein-isothiocyanate-conjugated secondary antibodies. Enzyme and fluorescence label was present in typical myrosin cells both in radicles and in cotyledons. With higher magnification, fluorescence label revealed that the intracellular localization of myrosinase was associated with the tonoplast-like membrane surrounding the myrosin grains in the myrosin cells. The results also indicate that a large proportion of the positive myrosin cells are located in the second-outermost cell layer of the peripheral cortex region of the radicles.

Page Thumbnails

  • Thumbnail: Page 
[245]
    [245]
  • Thumbnail: Page 
246
    246
  • Thumbnail: Page 
247
    247
  • Thumbnail: Page 
248
    248