You are not currently logged in.
Access JSTOR through your library or other institution:
Detoxification of xenobiotics by plant cells: characterisation of vacuolar amphiphilic organic anion transporters
Mechteld M.A. Blake-Kalff and Julian O.D. Coleman
Vol. 200, No. 4 (1996), pp. 426-431
Published by: Springer
Stable URL: http://www.jstor.org/stable/23384919
Page Count: 6
You can always find the topics here!Topics: Vacuoles, Barley, Cell membranes, Organic anion transporters, Bile acids, Tonoplast, Plant cells, Liver, Anions, Multiple drug resistance
Were these topics helpful?See somethings inaccurate? Let us know!
Select the topics that are inaccurate.
Preview not available
The transport activities of two primary ATP-dependent organic-anion transporters in the tonoplast of isolated barley (Hordeum vulgare L. cv. Klaxon) vacuoles have been characterised with N-ethylmaleimide glutathione (NEM-SG) and taurocholate as substrates. The transporters showed different sensitivities to organic anions and a variety of transport inhibitors and drugs. The vacuolar uptake of NEM-SG was inhibited by carbonylcyanide 4-trifluoromethoxyphenylhydrazone, 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid (DIDS), S-(2,4-dinitrophenyl)glutathione, alkyl-S-glutathione derivatives and taurocholate but stimulated by probenecid. The uptake of taurocholate was inhibited by vinblastine, DIDS and probenecid. Both transporters were unaffected by verapamil. The kinetic properties of the transporters indicate a general preference for amphiphilic anions with some substrate overlap. These characteristics of the transporters are similar to those displayed by the multidrug resistance protein of mammalian drug-resistant cells. We suggest that these vacuolar transporters be described as plant multispecific organic anion transporters (pMOATs).
Planta © 1996 Springer