You are not currently logged in.
Access JSTOR through your library or other institution:
Characterisation of pea cytosolic glutathione reductase expressed in transgenic tobacco
Rebecca G. Stevens, Gary P. Creissen and Philip M. Mullineaux
Vol. 211, No. 4 (September 2000), pp. 537-545
Published by: Springer
Stable URL: http://www.jstor.org/stable/23386070
Page Count: 9
You can always find the topics here!Topics: Peas, Protein isoforms, Chloroplasts, Enzymes, Gels, Plants, Transgenic plants, Complementary DNA, Fractionation, Leaves
Were these topics helpful?See somethings inaccurate? Let us know!
Select the topics that are inaccurate.
Preview not available
Expression in transgenic tobacco (Nicotiana tabacum L.) of a pea (Pisum sativum L.) GOR2 cDNA, encoding an isoform of glutathione reductase (GOR2), resulted in a 3- to 7-fold elevation of total foliar glutathione reductase (GR) activity. The enzyme encoded by GOR2 was confirmed to be extraplastidial in organelle fractionation studies and was considered most likely to be localised in the cytosol. A partial purification of GOR2 was achieved but a standard affinity chromatography step, using adenosine-2′,5′-diphosphate-Sepharose and often employed in the purification of GR from diverse sources, was unsuccessful with this isoform. Preparative isoelectric focussing was employed as part of the purification procedure of GOR2 and a complete separation from plastidial/mitochondrial glutathione reductase (GOR1) was achieved. The isoform GOR2 was shown to have a slower migration on non-denaturing polyacrylamide gels compared with GOR1 and properties typical of GR enzymes from plant sources.
Planta © 2000 Springer