You are not currently logged in.
Access JSTOR through your library or other institution:
Establishment and maintenance of friable, embryogenic maize callus and the involvement of L-proline
C.L. Armstrong and C.E. Green
Vol. 164, No. 2 (1985), pp. 207-214
Published by: Springer
Stable URL: http://www.jstor.org/stable/23388513
Page Count: 8
You can always find the topics here!Topics: Embryos, Plants, Callus, Corn, Immatures, Somatic embryogenesis, Sustainable agriculture, Nitrogen, Sustainable development, Tissue culture techniques
Were these topics helpful?See something inaccurate? Let us know!
Select the topics that are inaccurate.
Preview not available
Friable, embryogenic maize (Zea mays L.), inbred line A188, callus was established and maintained for more than one year without apparent loss of friability or embryogenic potential. Embryoid development was abundant in these cultures and plants were easily regenerated. Frequencies of friable-callus initiation and somatic-embryoid formation increased linearly with addition to N6 medium (C.C. Chu et al. 1975, Sci. Sin. [Peking] 18, 659—668) of up to 25 mM L-proline. Proline additions up to 9 mM to MS medium (inorganic elements of T. Murashige and F. Skoog 1962, Physiol. Plant. 15, 473—497, plus 0.5 mg l-1 thiamine hydrochloride and 150 mg l-1 L-asparagine monohydrate) did not stimulate embryoid formation. A major part of the difference between MS and N6 media could be attributed to their respective inorganic nitrogen components. L-Glutamine was not a satisfactory substitute for L-proline. Of 111 regenerated plants grown to maturity from three independent friable, embryogenic cell lines ranging in age from three to seven months, only four plants were abnormal based on morphology and pollen sterility. Seed was produced by 77% of the regenerated plants.
Planta © 1985 Springer