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DNA methylation increases throughout Arabidopsis development

L. Ruiz-García, M. T. Cervera and J. M. Martínez-Zapater
Planta
Vol. 222, No. 2 (October 2005), pp. 301-306
Published by: Springer
Stable URL: http://www.jstor.org/stable/23389045
Page Count: 6
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DNA methylation increases throughout Arabidopsis development
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Abstract

We used amplified fragment length polymorphisms (AFLP) to analyze the stability of DNA methylation throughout Arabidopsis development. AFLP can detect genome-wide changes in cytosine methylation produced by DNA demethylation agents, such as 5-azacytidine, or specific mutations at the DDM1 locus. In both cases, cytosine demethylation is associated with a general increase in the presence of amplified fragments. Using this approach, we followed DNA methylation at methylation sensitive restriction sites throughout Arabidopsis development. The results show a progressive DNA methylation trend from cotyledons to vegetative organs to reproductive organs.

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