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Human Transferrin: cDNA Characterization and Chromosomal Localization
Funmei Yang, J. B. Lum, John R. McGill, Charleen M. Moore, Susan L. Naylor, Peter H. Van Bragt, W. David Baldwin and Barbara H. Bowman
Proceedings of the National Academy of Sciences of the United States of America
Vol. 81, No. 9, [Part 1: Biological Sciences] (May 1, 1984), pp. 2752-2756
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/23412
Page Count: 5
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Transferrin (Tf) is the major iron binding protein in vertebrate serum. It shares homologous amino acid sequences with four other proteins: lactotransferrin, ovotransferrin, melanoma antigen p97, and HuBlym-1. Antigen p97 and the Tf receptor genes have been mapped on human chromosome 3. The goal of the study described here was to initiate the characterization of the Tf gene by identifying and characterizing its cDNA and mapping its chromosomal location. Recombinant plasmids containing human cDNA encoding Tf have been isolated by screening an adult human liver library with a mixed oligonucleotide probe. Within the 2.3 kilobase pairs of Tf cDNA analyzed, there is a probable leader sequence encoded by 57 nucleotides followed by 2037 nucleotides that encode the homologous amino and carboxyl domains. During evolution, three areas of the homologous amino and carboxyl domains have been strongly conserved, possibly reflecting functional constraints associated with iron binding. Chromosomal mapping by in situ hybridization and somatic cell hybrid analysis indicate that the Tf gene is located at q21-25 on human chromosome 3, consistent with linkage of the Tf, Tf receptor, and melanoma p97 loci.
Proceedings of the National Academy of Sciences of the United States of America © 1984 National Academy of Sciences