You are not currently logged in.
Access your personal account or get JSTOR access through your library or other institution:
If You Use a Screen ReaderThis content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Non-Cell-Autonomous Postmortem Lignification of Tracheary Elements in Zinnia elegans
Edouard Pesquet, Bo Zhang, András Gorzsás, Tuula Puhakainen, Henrik Serk, Sacha Escamez, Odile Barbier, Lorenz Gerber, Charleen Courtois-Moreau, Edward Alatalo, Lars Paulin, Jaakko Kangasjärvi, Björn Sundberg, Deborah Goffner and Hannele Tuominen
The Plant Cell
Vol. 25, No. 4 (APRIL 2013), pp. 1314-1328
Published by: American Society of Plant Biologists (ASPB)
Stable URL: http://www.jstor.org/stable/23483263
Page Count: 15
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Preview not available
Postmortem lignification of xylem tracheary elements (TEs) has been debated for decades. Here, we provide evidence in Zinnia elegans TE cell cultures, using pharmacological inhibitors and in intact Z. elegans plants using Fourier transform infrared microspectroscopy, that TE lignification occurs postmortem (i.e., after TE programmed cell death). In situ RT-PCR verified expression of the lignin monomer biosynthetic cinnamoyl CoA reductase and cinnamyl alcohol dehydrogenase in not only the lignifying TEs but also in the unlignified non-TE cells of Z. elegans TE cell cultures and in living, parenchymatic xylem cells that surround TEs in stems. These cells were also shown to have the capacity to synthesize and transport lignin monomers and reactive oxygen species to the cell walls of dead TEs. Differential gene expression analysis in Z. elegans TE cell cultures and concomitant functional analysis in Arabidopsis thaliana resulted in identification of several genes that were expressed in the non-TE cells and that affected lignin chemistry on the basis of pyrolysis—gas chromatography/mass spectrometry analysis. These data suggest that living, parenchymatic xylem cells contribute to TE lignification in a non-cell-autonomous manner, thus enabling the postmortem lignification of TEs.
The Plant Cell © 2013 American Society of Plant Biologists (ASPB)