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Desenvolvimento inicial de Pleopeltis lepidopteris (Polypodiaceae—Polypodiopsidia)

Daniela Viviani, Marisa Santos and Áurea Maria Randi
Rodriguésia
Vol. 60, No. 4 (2009), pp. 735-746
Stable URL: http://www.jstor.org/stable/23500310
Page Count: 12
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Desenvolvimento inicial de Pleopeltis lepidopteris (Polypodiaceae—Polypodiopsidia)
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Abstract

( Frondes férteis de Pleopeltis lepidopteris (Langsd. & Fisch.) de la Sota foram coletadas em Florianópolis, SC. Esporos foram separados dos esporângios por filtragem em papel entretela e armazenados a 7 ± 1°C, esterilizados superficialmente e inoculados em frascos contendo 20 ml de meio mineral proposto por Mohr e modificado por Dyer, suplementado por Benlate® (25 mg.l-1). A germinação ocorreu em sala de cultivo a 25 ± 2°C (30mmol m-2s-1) e fotoperíodo de 16 horas. Esporos de P. lepidopteris foram considerados potencialmente viáveis quando totalmente preenchidos com substâncias de coloração amarela. Os esporos são monoletes, com aproximadamente 40 × 62 μm, apresentando depósitos esféricos na superfície do perisporo papilada-granulada. Após 15 dias de inoculação de esporos, os gametófitos filamentosos apresentam uma célula rizoidal alongada, aclorofilada, uma fileira de quatro a sete células clorofiladas. Aos 30 dias a fase laminar espatulada está mais evidente e mostra um meristema apical central; após 45 dias os gametófitos são laminares e cordiformes; e após 120 dias de cultivo, o meristema apical é nítido enquanto tricomas unicelulares e estruturas reprodutivas são mais evidentes. Foi estudada a morfoanatomia de esporófitos de P. lepidopteris obtidos após a inoculação de esporos. A morfoanatomia das frondes de esporófitos jovens mostra que essa espécie apresenta escamas em ambas as faces das frondes, epiderme uniestratificada e mesofilo constituído por parênquima esponjoso. Estômatos do tipo anomocítico ocorrem na face abaxial e as células subsidiárias possuem paredes anticlinais sinuosas. O feixe vascular da raque é anficrival delimitado por endoderme. Spores of Pleopeltis lepidopteris (Langsd. & Fisch.) de la Sota were removed from fertile fronds collected in the isle of Santa Catarina, Brazil, separated from debris by filtering through lens paper and stored in glass jars at 7 ± 1°C. Superficially sterilized spores were sown in bottles containing 20 ml of mineral medium as proposed by Mohr, modified by Dyer and supplemented with Benomyl (25 mg.l-1). The germination was conducted in growth room, at 25 ± 2°C (30 mmol m-2s-1) and a 16 h-photoperiod. Spores of Pleopeltis lepidopteris considered to be viable were totally filled of yellow substances. The spores are monolete, with approximately 40 × 62 μm, and show spherical deposits in the smoothly papillate-granulate perispore. After 15 days of spore inoculation, the filamentous gametophyte present a long rhizoidal cell, without chlorophyll, a line of four to seven chlorophyllated cells; after 30 days, the spatulated laminar phase is more evident, and show a central apical meristem; after 45 days, the gametophyte are laminar and present the heart shape; after 120 days of culture the apical meristem is conspicuous; unicellular trichomes and reproductive structures are very evident. The morphoanatomy of the leaves of young sporophyte show scales in the frond faces, unistratified epidermis, the mesophyll consisted of a palisade and spongy parenchyma. The anomocytic stomata are presented in the abaxial frond face and the subsidiary cells show sinuous anticlinal walls. The rachis vascular anficrival tissue is delimited by the endoderm.

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