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Possible Role of the Golgi Apparatus in the Assembly of Very Low Density Lipoprotein
Mark J. Bamberger and M. Daniel Lane
Proceedings of the National Academy of Sciences of the United States of America
Vol. 87, No. 7 (Apr., 1990), pp. 2390-2394
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/2354227
Page Count: 5
You can always find the topics here!Topics: Hepatocytes, Triglycerides, Cytosol, Lipids, Secretion, Homogenization, Organelles, Secretory pathway, Incubation, Lipoproteins
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Transit of newly synthesized triacyl[3H]-glycerol through organelles of the sectetory system leading to assembly into nascent very low density lipoproteins (VLDLs) or to cytoplasmic storage was investigated in chick hepatocytes. Cells in monolayer culture were pulse-labeled with [2-3H]glycerol, and after different periods of chase with unlabeled glycerol, the movement of triacyl [3H]glycerol through the endoplasmic reticulum (ER) and Golgi and the incorporation into nascent VLDL and cytoplasmic triacylglycerol-rich vesicles (TGRVs) were determined. Initially, newly synthesized triacyl [3H]glycerol is tightly associated with the ER. Movement from the ER of triacyl [3H]glycerol destined for cytoplasmic storage (as TGRVs) is extremely rapid and virtually complete within 8 min of chase. After 8 min of chase, triacyl [3H]glycerol lost from organelles of the secretory system was accounted for entirely as triacyl [3H]glycerol secreted as VLDL. Comparison of rates of movement of triacyl [3H]glycerol, apolipoprotein B, apolipoprotein II, and apolipoprotein A-I through the ER and Golgi and of their secretion in nascent VLDL suggests that assembly of triacylglcerol with apolipoproteins occurs in the Golgi. Experiment with permeabilized hepatocytes supplemented with cytosol show that newly synthesized triacyl [3H]-glycerol and [3H]phospholipid moves from the ER through the full-density range of Giogi elements and is dependent upon supplementary ATP.
Proceedings of the National Academy of Sciences of the United States of America © 1990 National Academy of Sciences