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Direct Determination of Phospholipid Lamellar Structure at 0.34-nm Resolution
Douglas L. Dorset, Erich Beckmann and Friedrich Zemlin
Proceedings of the National Academy of Sciences of the United States of America
Vol. 87, No. 19 (Oct., 1990), pp. 7570-7573
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/2355272
Page Count: 4
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Low-dose, high-resolution electron microscopy combined with conventional direct-phasing methods based on the estimates of triplet-structure invariants are used to determine phase values for all observed electron-diffraction-structure factor magnitudes from epitaxially oriented multilamellar paracrystals of the phosphospholipid 1,2-dihexadecyl-sn-glycerophosphoethanolamine. The reverse Fourier transform of these phased-structure factors is a one-dimensional electrostatic potential map that strongly resembles the electron-density maps calculated from similar x-ray-diffraction data. Determination of the phase values for the electron-diffraction data with structure invariants alone is nearly as successful as the combined use of two separate methods, assigning values to 13 of the 16 reflections--i.e., the electrostatic potential map closely resembles the one calculated with all data.
Proceedings of the National Academy of Sciences of the United States of America © 1990 National Academy of Sciences