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Wheat Dwarf Virus Ac/Ds Vectors: Expression and Excision of Transposable Elements Introduced into Various Cereals by a Viral Replicon
Jurgen Laufs, Uwe Wirtz, Matthias Kammann, Volker Matzeit, Sabine Schaefer, Jeff Schell, Armin Peter Czernilofsky, Barbara Baker and Bruno Gronenborn
Proceedings of the National Academy of Sciences of the United States of America
Vol. 87, No. 19 (Oct., 1990), pp. 7752-7756
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/2355309
Page Count: 5
You can always find the topics here!Topics: Genetic transposition, DNA, Genomes, Transfection, Transposons, Replicon, Plasmids, Viruses, Protoplasts, Polymerase chain reaction
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The maize transposon Activator (Ac) and in vitro-generated nonautonomous derivatives thereof [AcΔ or Dissociation (Ds) elements] were inserted into the genome of a geminivirus of graminaceous plants, wheat dwarf virus, at a site that does not interfere with viral replication. These recombinant viral genomes were introduced into wheat, maize, and rice protoplasts, where rapid and efficient excision of Ac was observed. Excision was detected only in vectors in which, after transfection, the virus could replicate. This result is not restricted to the autonomous Ac; excision of Ds elements was also induced by transposase activity provided in trans by plasmids expressing the cDNA of Ac. The potential of this combination of a transposon with a viral replicon for plant molecular genetic engineering is discussed.
Proceedings of the National Academy of Sciences of the United States of America © 1990 National Academy of Sciences