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A DNA Gyrase-Binding Site at the Center of the Bacteriophage Mu Genome is Required for Efficient Replicative Transposition
Martin L. Pato, Martha M. Howe and N. Patrick Higgins
Proceedings of the National Academy of Sciences of the United States of America
Vol. 87, No. 22 (Nov., 1990), pp. 8716-8720
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/2355617
Page Count: 5
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We have discovered a centrally located site that is required for efficient replication of bacteriophage Mu DNA and identified it as a strong DNA gyrase-binding site. Incubation of Mu DNA with gyrase and enoxacin revealed a cleavage site 18.1 kilobases from the left end of the 37.2-kilobase genome. Two observations indicate a role for the site in Mu replication: mutants of Mu, able to grow on an Escherichia coli gyrB host that does not allow growth of wild-type Mu, were found to possess single-base changes resulting in more efficient gyrase binding and cleavage at the site. Introduction of a 147-base-pair deletion that eliminated the site from a prophage inhibited the onset of Mu replication for >1 hr after induction.
Proceedings of the National Academy of Sciences of the United States of America © 1990 National Academy of Sciences