Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

A DNA Gyrase-Binding Site at the Center of the Bacteriophage Mu Genome is Required for Efficient Replicative Transposition

Martin L. Pato, Martha M. Howe and N. Patrick Higgins
Proceedings of the National Academy of Sciences of the United States of America
Vol. 87, No. 22 (Nov., 1990), pp. 8716-8720
Stable URL: http://www.jstor.org/stable/2355617
Page Count: 5
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
A DNA Gyrase-Binding Site at the Center of the Bacteriophage Mu Genome is Required for Efficient Replicative Transposition
Preview not available

Abstract

We have discovered a centrally located site that is required for efficient replication of bacteriophage Mu DNA and identified it as a strong DNA gyrase-binding site. Incubation of Mu DNA with gyrase and enoxacin revealed a cleavage site 18.1 kilobases from the left end of the 37.2-kilobase genome. Two observations indicate a role for the site in Mu replication: mutants of Mu, able to grow on an Escherichia coli gyrB host that does not allow growth of wild-type Mu, were found to possess single-base changes resulting in more efficient gyrase binding and cleavage at the site. Introduction of a 147-base-pair deletion that eliminated the site from a prophage inhibited the onset of Mu replication for >1 hr after induction.

Page Thumbnails

  • Thumbnail: Page 
8716
    8716
  • Thumbnail: Page 
8717
    8717
  • Thumbnail: Page 
8718
    8718
  • Thumbnail: Page 
8719
    8719
  • Thumbnail: Page 
8720
    8720