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The Use of Sarkosyl in Generating Soluble Protein After Bacterial Expression
Stewart Frankel, Regina Sohn and Leslie Leinwand
Proceedings of the National Academy of Sciences of the United States of America
Vol. 88, No. 4 (Feb. 15, 1991), pp. 1192-1196
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/2355960
Page Count: 5
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Actin, like many other proteins, is highly insoluble after expression in Escherichia coli. In order to understand the origin of insoluble aggregates, we asked whether morphological inclusions were always correlated with insolubility. The strain expressing actin was compared to one that expresses part of the myosin tail; the latter strain yields soluble protein after various cell lysis or disruption procedures. Morphological inclusions were observed in both strains, indicating there is no obligate relationship between solubility and inclusions. Studies presented here suggest that extreme insolubility results from coaggregation of the actin with bacterial outer membrane components upon bacterial lysis. The properties of the outer membrane have been exploited in the development of nondenaturing procedures that yield soluble actin. One procedure involves the disruption of coaggregates with sarkosyl detergent (N-laurylsarcosine); another prevents the formation of coaggregates by lysing in the presence of sarkosyl. These methods may be useful for other proteins that become insoluble after bacterial expression.
Proceedings of the National Academy of Sciences of the United States of America © 1991 National Academy of Sciences