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A Homeodomain Protein Binds to γ-Globin Gene Regulatory Sequences

Donald Lavelle, Janice Ducksworth, Eva Eves, Georgina Gomes, Michael Keller, Paul Heller and Joseph DeSimone
Proceedings of the National Academy of Sciences of the United States of America
Vol. 88, No. 16 (Aug. 15, 1991), pp. 7318-7322
Stable URL: http://www.jstor.org/stable/2357674
Page Count: 5
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
A Homeodomain Protein Binds to γ-Globin Gene Regulatory Sequences
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Abstract

Developmental regulation of γ-globin gene expression probably occurs through developmental-stage-specific trans-acting factors able to promote the interaction of enhancer elements located in the far upstream locus control region with regulatory elements in the γ gene promoter, and 3' Aγ enhancer located in close proximity to the genes. We have detected a nuclear protein in K562 and baboon fetal bone marrow nuclear extracts capable of binding to A+T-rich sequences in the locus control region, γ gene promoter, and 3' Aγ enhancer. SDS/polyacrylamide gel analysis of the purified K562 binding activity revealed a single protein of 87 kDa. A K562 cDNA clone was isolated encoding a β-galactosidase fusion protein with a DNA binding specificity identical to that of the K562/fetal bone marrow nuclear protein. The cDNA clone encodes a homeodomain homologous to the Drosophila antennapedia protein

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