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Cytoduction in Chlamydomonas reinhardtii
Rene F. Matagne, Claire Remacle and Monique Dinant
Proceedings of the National Academy of Sciences of the United States of America
Vol. 88, No. 16 (Aug. 15, 1991), pp. 7447-7450
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/2357701
Page Count: 4
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After conjugation between Chlamydomonas gametes of opposite mating type, a transient dikaryon is formed. The two nuclei fuse within 4-6 hr after mating. The young diploid zygote differentiates into dormant zygospore competent to complete meiosis, or more rarely (2-10% of cases) it undergoes mitosis to produce a stable diploid progeny. We here bring genetical, biochemical, and cytological evidence that among the mitotic zygotes, a large proportion of them undergo cytokinesis without fusion of the nuclei-a process that has been termed "cytoduction." By using appropriate genetic markers, haploid cytoductants that possess the nuclear genotype of one parent and the chloroplast marker of the other parent can easily be isolated. Genetical analysis and hybridization experiments moreover show that many haploid cytoductants transmit the chloroplast DNA molecules of both parents and that, as in diploids, these DNA copies occasionally recombine. This process of cytoduction extends the life cycle of Chlamydomonas and provides new tools for its genetic analysis.
Proceedings of the National Academy of Sciences of the United States of America © 1991 National Academy of Sciences