Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Cloning, Molecular Characterization, and Chromosomal Assignment of a Gene Encoding a Second D1 Dopamine Receptor Subtype: Differential Expression Pattern in Rat Brain Compared with the D1A Receptor

Mario Tiberi, Keith R. Jarvie, Chris Silvia, Pierre Falardeau, Jay A. Gingrich, Nathalie Godinot, Lucie Bertrand, Teresa L. Yang-Feng, Robert T. Fremeau, Jr. and Marc G. Caron
Proceedings of the National Academy of Sciences of the United States of America
Vol. 88, No. 17 (Sep. 1, 1991), pp. 7491-7495
Stable URL: http://www.jstor.org/stable/2357716
Page Count: 5
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Cloning, Molecular Characterization, and Chromosomal Assignment of a Gene Encoding a Second D1 Dopamine Receptor Subtype: Differential Expression Pattern in Rat Brain Compared with the D1A Receptor
Preview not available

Abstract

Multiple D1 dopaminergic receptor subtypes have been postulated on the basis of pharmacological, biochemical, and genetic studies. We describe the isolation and characterization of a rat gene encoding a dopamine receptor that is structurally and functionally similar to the D1 dopamine receptor. The coding region, which is intronless, encodes a protein of 475 amino acids (Mr 52,834) with structural features that are consistent with receptors coupled to guanine nucleotide-binding regulatory proteins. The expressed protein binds dopaminergic ligands and mediates stimulation of adenylyl cyclase with pharmacological properties similar to those of the D1 dopamine receptor. The gene encoding the human homologue of this receptor subtype is located to the short arm of chromosome 4 (4p16.3), the same region as the Huntington disease gene. In striking contrast to the previously cloned D1 receptor, little or no mRNA for the receptor described here was observed in striatum, nucleus accumbens, olfactory tubercle, and frontal cortex. High levels of mRNA for this receptor were found in distinct layers of the hippocampus, the mammillary nuclei, and the anterior pretectal nuclei, brain regions that have been shown to exhibit little or no D1 dopamine receptor binding. On the basis of its properties we propose that this dopamine receptor subtype be called D1B.

Page Thumbnails

  • Thumbnail: Page 
7491
    7491
  • Thumbnail: Page 
7492
    7492
  • Thumbnail: Page 
7493
    7493
  • Thumbnail: Page 
7494
    7494
  • Thumbnail: Page 
7495
    7495