Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

A Method for Production of Antibodies to Human T-Cell Receptor β-Chain Variable Regions

Yongwon Choi, Brian Kotzin, Joyce Lafferty, Janice White, Michele Pigeon, Ralph Kubo, John Kappler and Philippa Marrack
Proceedings of the National Academy of Sciences of the United States of America
Vol. 88, No. 19 (Oct. 1, 1991), pp. 8357-8361
Stable URL: http://www.jstor.org/stable/2357938
Page Count: 5
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
A Method for Production of Antibodies to Human T-Cell Receptor β-Chain Variable Regions
Preview not available

Abstract

Mouse T-cell hybridomas bearing human Vβ elements were produced by transfection of human/mouse hybrid T-cell receptor β-chain genes into a mouse T-cell hybridoma lacking an endogenous β-chain gene. These hybridomas were entirely mouse in origin except for the human Vβ region. These cells were used to immunize mice against human Vβ elements. Mouse monoclonal antibodies have thus been generated against human Vβ13.1 and -13.2. We expect that the method outlined in this paper will be useful in the production of monoclonal antibodies specific for other human Vβ or Vα elements.

Page Thumbnails

  • Thumbnail: Page 
8357
    8357
  • Thumbnail: Page 
8358
    8358
  • Thumbnail: Page 
8359
    8359
  • Thumbnail: Page 
8360
    8360
  • Thumbnail: Page 
8361
    8361