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A Method for Production of Antibodies to Human T-Cell Receptor β-Chain Variable Regions
Yongwon Choi, Brian Kotzin, Joyce Lafferty, Janice White, Michele Pigeon, Ralph Kubo, John Kappler and Philippa Marrack
Proceedings of the National Academy of Sciences of the United States of America
Vol. 88, No. 19 (Oct. 1, 1991), pp. 8357-8361
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/2357938
Page Count: 5
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Mouse T-cell hybridomas bearing human Vβ elements were produced by transfection of human/mouse hybrid T-cell receptor β-chain genes into a mouse T-cell hybridoma lacking an endogenous β-chain gene. These hybridomas were entirely mouse in origin except for the human Vβ region. These cells were used to immunize mice against human Vβ elements. Mouse monoclonal antibodies have thus been generated against human Vβ13.1 and -13.2. We expect that the method outlined in this paper will be useful in the production of monoclonal antibodies specific for other human Vβ or Vα elements.
Proceedings of the National Academy of Sciences of the United States of America © 1991 National Academy of Sciences