Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Reconstitution of Tracheal Grafts with a Genetically Modified Epithelium

John F. Engelhardt, Edward D. Allen and James M. Wilson
Proceedings of the National Academy of Sciences of the United States of America
Vol. 88, No. 24 (Dec. 15, 1991), pp. 11192-11196
Stable URL: http://www.jstor.org/stable/2359210
Page Count: 5
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Reconstitution of Tracheal Grafts with a Genetically Modified Epithelium
Preview not available

Abstract

A rational approach to the development of gene therapies for cystic fibrosis requires a better understanding of the cellular targets for gene transfer in the airway epithelium. We have used recombinant retroviruses to study the dynamics and lineage relationships of a regenerating rat tracheal epithelium. Primary cultures of tracheal epithelial cells were exposed to lacZ-transducing retroviruses and subsequently seeded into denuded trachea that were implanted into BALB/c (nu/nu) mice. The grafts developed a fully differentiated mucociliary epithelium containing large clones of lacZ-expressing cells with virtually all cell types represented within each clone. These data are most consistent with gene transfer into a putative progenitor cell that is capable of extensive self renewal and pleuripotent development. Vector-specific variation in transgene expression was noted in the various cell types.

Page Thumbnails

  • Thumbnail: Page 
11192
    11192
  • Thumbnail: Page 
11193
    11193
  • Thumbnail: Page 
11194
    11194
  • Thumbnail: Page 
11195
    11195
  • Thumbnail: Page 
11196
    11196