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Possible Involvement of the Long Terminal Repeat of Transposable Element 17.6 in Regulating Expression of an Insecticide Resistance-Associated P450 Gene in Drosophila
Larry C. Waters, Andrew C. Zelhof, Brenda J. Shaw and Lan-Yang Ch'ang
Proceedings of the National Academy of Sciences of the United States of America
Vol. 89, No. 11 (Jun. 1, 1992), pp. 4855-4859
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/2359545
Page Count: 5
You can always find the topics here!Topics: Complementary DNA, Messenger RNA, Genes, Drosophila, DNA, Genomics, Signals, Amino acids, Enzymes, Resistance mechanisms
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P450-A and P450-B are electrophoretically defined subsets of cytochrome P450 enzymes in Drosophila melanogaster. P450-A is present among all strains tested, whereas expression of P450-B is associated with resistance to insecticides. Monoclonal antibodies were used to obtain cDNA clones for an enzyme from each P450 subset (i.e., P450-A1 and P450-B1). The P450-B1 cDNA was sequenced and shown to code for a P450 of 507 amino acids. Its gene has been named CYP6A2. Comparative molecular analyses of a pair of susceptible, 91-C, and resistant, 91-R, Drosophila strains were made. There was 20-30 times more P450-B1 mRNA in 91-R than in 91-C, and the small amount of P450-B1 mRNA in 91-C was significantly larger in size than that in 91-R. The P450-B1 gene in 91-R was structurally different from that in 91-C but was not amplified. The P450-B1 gene in 91-C contained a solitary long terminal repeat of transposable element 17.6 in its 3' untranslated region. It was absent in the P450-B1 gene of 91-R. On the basis of features of the long terminal repeat and its location in the gene of the susceptible fly, we propose that a posttranscriptional mechanism involving mRNA stability could be involved in regulating P450-B1 gene expression.
Proceedings of the National Academy of Sciences of the United States of America © 1992 National Academy of Sciences