Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Gene Networks and Chromatin and Transcriptional Regulation of the Phaseolin Promoter in Arabidopsis

Sabarinath Sundaram, Sunee Kertbundit, Eugene V. Shakirov, Lakshminarayan M. Iyer, Miloslav Juříček and Timothy C. Hall
The Plant Cell
Vol. 25, No. 7 (JULY 2013), pp. 2601-2617
Stable URL: http://www.jstor.org/stable/23598296
Page Count: 17
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Gene Networks and Chromatin and Transcriptional Regulation of the Phaseolin Promoter in Arabidopsis
Preview not available

Abstract

The complete lack of seed storage protein expression in vegetative tissues and robust expression during embryogenesis makes seed development an ideal system to study tissue-specific expression of genes. The promoter for the Phaseolin (phas) gene, which encodes the major seed storage protein in bean (Phaseolus vulgaris), is activated in two sequential steps: Phaseolus vulgaris ABI3-like factor (Pv-ALF)—dependent potentiation and abscisic acid—mediated activation. In this study, a heterologous in vivo Pv-ALF/phas-GUS (for β-glucuronidase) expression system in transgenic Arabidopsis thaliana leaves was used in conjunction with the powerful RNA-Seq approach to capture transcriptional landscapes of phas promoter expression. Remarkably, expression of over 1300 genes from 11 functional categories coincided with changes in the transcriptional status of the phas promoter. Gene network analysis of induced genes and artificial microRNA—mediated loss-of-function genetic assays identified transcriptional regulators RINGLET 2 (RLT2) and AINTEGUMENTA-LIKE 5 (AIL5) as being essential for phas transcription. Pv-ALF binding to the RLT2 and AIL5 promoter regions was confirmed by electrophoretic mobility shift assay. RLT2 and AIL5 knockdown lines displayed reduced expression of several endogenous seed genes, suggesting that these factors are involved in activation of endogenous Arabidopsis seed storage gene expression. Overall, the identification of these key factors involved in phas activation provides important insight into the two-step transcriptional regulation of seed-specific gene expression.

Page Thumbnails

  • Thumbnail: Page 
[2601]
    [2601]
  • Thumbnail: Page 
2602
    2602
  • Thumbnail: Page 
2603
    2603
  • Thumbnail: Page 
2604
    2604
  • Thumbnail: Page 
2605
    2605
  • Thumbnail: Page 
2606
    2606
  • Thumbnail: Page 
2607
    2607
  • Thumbnail: Page 
2608
    2608
  • Thumbnail: Page 
2609
    2609
  • Thumbnail: Page 
2610
    2610
  • Thumbnail: Page 
2611
    2611
  • Thumbnail: Page 
2612
    2612
  • Thumbnail: Page 
2613
    2613
  • Thumbnail: Page 
2614
    2614
  • Thumbnail: Page 
2615
    2615
  • Thumbnail: Page 
2616
    2616
  • Thumbnail: Page 
2617
    2617