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Genome-Wide Binding Analysis of the Transcription Activator IDEAL PLANT ARCHITECTURE1 Reveals a Complex Network Regulating Rice Plant Architecture
Zefu Lu, Hong Yu, Guosheng Xiong, Jing Wang, Yongqing Jiao, Guifu Liu, Yanhui Jing, Xiangbing Meng, Xingming Hu, Qian Qian, Xiangdong Fu, Yonghong Wang and Jiayang Li
The Plant Cell
Vol. 25, No. 10 (OCTOBER 2013), pp. 3743-3759
Published by: American Society of Plant Biologists (ASPB)
Stable URL: http://www.jstor.org/stable/23598430
Page Count: 17
You can always find the topics here!Topics: Rice, Genes, Binding sites, Panicles, Gene expression regulation, Plant architecture, Plant cells, Plants, Promoter regions, Tillers
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IDEAL PLANT ARCHITECTURE1 (IPA1) is critical in regulating rice (Oryza sativa) plant architecture and substantially enhances grain yield. To elucidate its molecular basis, we first confirmed IPA1 as a functional transcription activator and then identified 1067 and 2185 genes associated with IPA1 binding sites in shoot apices and young panicles, respectively, through chromatin immunoprecipitation sequencing assays. The SQUAMOSA PROMOTER BINDING PROTEIN-box direct binding core motif GTAC was highly enriched in IPA1 binding peaks; interestingly, a previously uncharacterized indirect binding motif TGGGCC/T was found to be significantly enriched through the interaction of IPA1 with proliferating cell nuclear antigen PROMOTER BINDING FACTOR1 or PROMOTER BINDING FACTOR2. Genome-wide expression profiling by RNA sequencing revealed IPA1 roles in diverse pathways. Moreover, our results demonstrated that IPA1 could directly bind to the promoter of rice TEOSINTE BRANCHED1, a negative regulator of tiller bud outgrowth, to suppress rice tillering, and directly and positively regulate DENSE AND ERECT PANICLE1, an important gene regulating panicle architecture, to influence plant height and panicle length. The elucidation of target genes of IPA1 genome-wide will contribute to understanding the molecular mechanisms underlying plant architecture and to facilitating the breeding of elite varieties with ideal plant architecture.
The Plant Cell © 2013 American Society of Plant Biologists (ASPB)