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Cloned and Expressed Macrophage Nitric Oxide Synthase Contrasts with the Brain Enzyme

Charles J. Lowenstein, Charles S. Glatt, David S. Bredt and Solomon H. Snyder
Proceedings of the National Academy of Sciences of the United States of America
Vol. 89, No. 15 (Aug. 1, 1992), pp. 6711-6715
Stable URL: http://www.jstor.org/stable/2359859
Page Count: 5
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Cloned and Expressed Macrophage Nitric Oxide Synthase Contrasts with the Brain Enzyme
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Abstract

Nitric oxide (NO) is a messenger molecule of macrophages, endothelial cells in blood vessels, and neurons. A neuronal form of NO synthase (NOS) has been previously cloned. We now report the molecular cloning of macrophage NOS. The macrophage enzyme displays 50% sequence identity to the neuronal enzyme. Like neuronal NOS, macrophage NOS has recognition sites for FAD, FMN, and NADPH and also has a consensus calmodulin binding site. Macrophage NOS mRNA is strikingly inducible; it is absent in quiescent macrophages or spleen but is prominent 2-6 hr after endotoxin treatment.

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