Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

GALACTURONOSYLTRANSFERASE-LIKE5 Is Involved in the Production of Arabidopsis Seed Coat Mucilage

Yingzhen Kong, Gongke Zhou, Ashraf A. Abdeen, James Schafhauser, Beth Richardson, Melani A. Atmodjo, Jiyoung Jung, Louise Wicker, Debra Mohnen, Tamara Western and Michael G. Hahn
Plant Physiology
Vol. 163, No. 3 (November 2013), pp. 1203-1217
Stable URL: http://www.jstor.org/stable/23598622
Page Count: 15
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
GALACTURONOSYLTRANSFERASE-LIKE5 Is Involved in the Production of Arabidopsis Seed Coat Mucilage
Preview not available

Abstract

The function of a putative galacturonosyltransferase from Arabidopsis (Arabidopsis thaliana; At1g02720; GALACTURONOSYLTRANSFERASE-LIKE5 [AtGATL5]) was studied using a combination of molecular genetic, chemical, and immunological approaches. AtGATL5 is expressed in all plant tissues, with highest expression levels in siliques 7 DPA. Furthermore, its expression is positively regulated by several transcription factors that are known to regulate seed coat mucilage production. AtGATL5 is localized in both endoplasmic reticulum and Golgi, in comparison with marker proteins resident to these subcellular compartments. A transfer DNA insertion in the AtGATL5 gene generates seed coat epidermal cell defects both in mucilage synthesis and cell adhesion. Transformation of atgatl5-1 mutants with the wild-type AtGATL5 gene results in the complementation of all morphological phenotypes. Compositional analyses of the mucilage isolated from the atgatl5-1 mutant demonstrated that galacturonic acid and rhamnose contents are decreased significantly in atgatl5-1 compared with wild-type mucilage. No changes in structure were observed between soluble mucilage isolated from wild-type and mutant seeds, except that the molecular weight of the mutant mucilage increased 63% compared with that of the wild type. These data provide evidence that AtGATL5 might function in the regulation of the final size of the mucilage rhamnogalacturonan I.

Page Thumbnails

  • Thumbnail: Page 
1203
    1203
  • Thumbnail: Page 
1204
    1204
  • Thumbnail: Page 
1205
    1205
  • Thumbnail: Page 
1206
    1206
  • Thumbnail: Page 
1207
    1207
  • Thumbnail: Page 
1208
    1208
  • Thumbnail: Page 
1209
    1209
  • Thumbnail: Page 
1210
    1210
  • Thumbnail: Page 
1211
    1211
  • Thumbnail: Page 
1212
    1212
  • Thumbnail: Page 
1213
    1213
  • Thumbnail: Page 
1214
    1214
  • Thumbnail: Page 
1215
    1215
  • Thumbnail: Page 
1216
    1216
  • Thumbnail: Page 
1217
    1217