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High-Level Expression of a Heterologous Protein in the Milk of Transgenic Swine Using the cDNA Encoding Human Protein C
William H. Velander, John L. Johnson, Raymond L. Page, Christopher G. Russell, Anuradha Subramanian, Tracy D. Wilkins, Francis C. Gwazdauskas, Christoph Pittius and William N. Drohan
Proceedings of the National Academy of Sciences of the United States of America
Vol. 89, No. 24 (Dec. 15, 1992), pp. 12003-12007
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/2360845
Page Count: 5
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Transgenic pigs were generated that produced human protein C in their milk at up to 1 g/liter. The gene construct was a fusion gene consisting of the cDNA for human protein C inserted into the first exon of the mouse whey acidic protein gene. These results demonstrate that the mouse whey acidic protein gene contains regulatory elements that can direct cDNA expression at high levels in the pig mammary gland. Recombinant human protein C that was produced at about 380 μg/ml per hr in transgenic pig milk possessed anticoagulant activity that was equivalent to that of protein C derived from human plasma. These studies provide evidence that γ-carboxylation can occur at high levels in the mammary gland of a pig.
Proceedings of the National Academy of Sciences of the United States of America © 1992 National Academy of Sciences