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The Bovine Papillomavirus Origin of Replication Requires a Binding Site for the E2 Transcriptional Activator
Ene Ustav, Mart Ustav, Paul Szymanski and Arne Stenlund
Proceedings of the National Academy of Sciences of the United States of America
Vol. 90, No. 3 (Feb. 1, 1993), pp. 898-902
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/2360923
Page Count: 5
You can always find the topics here!Topics: Binding sites, DNA, Plasmids, Cell lines, DNA replication, CHO cells, COS cells, Dimerization, Replication origin, Ungulates
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The bovine papillomavirus type I transcriptional activator E2 is essential for replication of bovine papillomavirus DNA, yet most of the high-affinity binding sites for E2 are dispensable. Here we demonstrate an absolute requirement for a binding site for the E2 polypeptide as a cis-acting replication element, establishing that site-specific binding of E2 to the origin is a prerequisite for bovine papillomavirus replication in vivo. The position and distance of the E2 binding site relative to the other origin of replication components are flexible, but function at a distance requires high-affinity E2 binding sites. Thus, low-affinity binding sites function only when located close to the origin of replication, while activity at greater distances requires multimerized high-affinity E2 binding sites. The requirement for E2, although different in some respects, shows distinct similarities to what has been termed replication enhancers and may provide insight into the function of this class of DNA replication element.
Proceedings of the National Academy of Sciences of the United States of America © 1993 National Academy of Sciences