Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Gene 5.5 Protein of Bacteriophage T7 Inhibits the Nucleoid Protein H-NS of Escherichia coli

Qingyun Liu and Charles C. Richardson
Proceedings of the National Academy of Sciences of the United States of America
Vol. 90, No. 5 (Mar. 1, 1993), pp. 1761-1765
Stable URL: http://www.jstor.org/stable/2361397
Page Count: 5
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Gene 5.5 Protein of Bacteriophage T7 Inhibits the Nucleoid Protein H-NS of Escherichia coli
Preview not available

Abstract

Gene 5.5 of coliphage T7 is one of the most highly expressed genes during T7 infection. Gene 5.5 protein, purified from cells overexpressing the cloned gene, purifies with the nucleoid protein H-NS of Escherichia coli during three chromatographic steps. A fusion protein of gene 5.5 protein and maltose binding protein also purifies with H-NS. The fusion protein binds to the DNA-H-NS complex and abolishes H-NS-mediated inhibition of transcription by Escherichia coli and T7 RNA polymerases in vitro. Expression of gene 5.5 also relieves the repression of the Escherichia coli proU promoter by H-NS in vivo. The change of leucine to proline at residue 30 of gene 5.5 protein abolishes the interaction between gene 5.5 protein and H-NS.

Page Thumbnails

  • Thumbnail: Page 
1761
    1761
  • Thumbnail: Page 
1762
    1762
  • Thumbnail: Page 
1763
    1763
  • Thumbnail: Page 
1764
    1764
  • Thumbnail: Page 
1765
    1765