Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Preferential Repair of Cyclobutane Pyrimidine Dimers in the Transcribed Strand of a Gene in Yeast Chromosomes and Plasmids is Dependent on Transcription

Kevin S. Sweder and Philip C. Hanawalt
Proceedings of the National Academy of Sciences of the United States of America
Vol. 89, No. 22 (Nov. 15, 1992), pp. 10696-10700
Stable URL: http://www.jstor.org/stable/2361977
Page Count: 5
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Preferential Repair of Cyclobutane Pyrimidine Dimers in the Transcribed Strand of a Gene in Yeast Chromosomes and Plasmids is Dependent on Transcription
Preview not available

Abstract

While preferential repair of the transcribed strands within active genes has been demonstrated in organisms as diverse as humans and Escherichia coli, it has not previously been shown to occur in chromosomal genes in the yeast Saccharomyces cerevisiae. We found that repair of cyclobutane pyrimidine dimers in the transcribed strand of the expressed RPB2 gene in the chromosome of a repair-proficient strain is much more rapid than that in the nontranscribed strand. Furthermore, a copy of the RPB2 gene borne on a centromeric ARS1 plasmid showed the same strand bias in repair. To investigate the relation of this strand bias to transcription, we studied repair in a yeast strain with the temperature-sensitive mutation, rpb1-1, in the largest subunit of RNA polymerase II. When exponentially growing rpb1-1 cells are shifted to the nonpermissive temperature, they rapidly cease mRNA synthesis. At the permissive temperature, both rpb1-1 and the wild-type, parental cells exhibited rapid, proficient repair in the transcribed strand of chromosomal and plasmid-borne copies of the RPB2 gene. At the nonpermissive temperature, the rate of repair in the transcribed strand in rpb1-1 cells was reduced to that in the nontranscribed strand. These findings establish the dependence of strand bias in repair on transcription by RNA polymerase II in the chromosomes and in plasmids, and they validate the use of plasmids for analysis of the relation of repair to transcription in yeast.

Page Thumbnails

  • Thumbnail: Page 
10696
    10696
  • Thumbnail: Page 
10697
    10697
  • Thumbnail: Page 
10698
    10698
  • Thumbnail: Page 
10699
    10699
  • Thumbnail: Page 
10700
    10700