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Dispensable Sequence Motifs in the RAG-1 and RAG-2 Genes for Plasmid V(D)J Recombination
Daniel P. Silver, Eugenia Spanopoulou, Richard C. Mulligan and David Baltimore
Proceedings of the National Academy of Sciences of the United States of America
Vol. 90, No. 13 (Jul. 1, 1993), pp. 6100-6104
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/2362338
Page Count: 5
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As a probe of whether RAG-1 and RAG-2 gene products activate other genes or form part of the recombinase itself, certain mutants of the RAG genes were assayed for their ability to activate variable-diversity-joining region [V(D)J] recombination in a plasmid substrate in fibroblasts. The results indicate that the N-terminal one-third of RAG-1, including a zinc-finger-like domain, and an acidic domain of RAG-2 are dispensable for activating V(D)J recombination in a fibroblast, although they contribute quantitatively. In contrast, deletion of the C-terminal segment of RAG-1, which has homology to a topoisomerase-like protein from yeast, abolished recombination activation. These results do not support the hypothesis that the RAG gene products are transcription factors and suggest the possibility that they are parts of the recombination machinery.
Proceedings of the National Academy of Sciences of the United States of America © 1993 National Academy of Sciences