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Multiple Nucleosome Positioning with Unique Rotational Setting for the Saccharomyces cerevisiae 5S rRNA Gene in vitro and in vivo
Memmo Buttinelli, Ernesto Di Mauro and Rodolfo Negri
Proceedings of the National Academy of Sciences of the United States of America
Vol. 90, No. 20 (Oct. 15, 1993), pp. 9315-9319
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/2363144
Page Count: 5
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A simple no-background assay was developed for high-resolution in vivo analysis of yeast chromatin. When applied to Saccharomyces cerevisiae 5S rRNA genes (5S rDNA), this analysis shows that nucleosomes completely cover this chromosomal region, occupying alternative positions characterized by a unique helical phase. This supports the notion that sequence-intrinsic rotational signals are the major determinant of nucleosome localization. Nucleosomal core particles reconstituted in vitro occupy the same positions and have the same helically phased distribution observed in vivo, as determined by mapping of exonuclease III-resistant borders, mapping by restriction cleavages, and by DNase I and hydroxyl-radical digestion patterns.
Proceedings of the National Academy of Sciences of the United States of America © 1993 National Academy of Sciences