You are not currently logged in.
Access JSTOR through your library or other institution:
If You Use a Screen ReaderThis content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Multiple Nucleosome Positioning with Unique Rotational Setting for the Saccharomyces cerevisiae 5S rRNA Gene in vitro and in vivo
Memmo Buttinelli, Ernesto Di Mauro and Rodolfo Negri
Proceedings of the National Academy of Sciences of the United States of America
Vol. 90, No. 20 (Oct. 15, 1993), pp. 9315-9319
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/2363144
Page Count: 5
You can always find the topics here!Topics: DNA, Nucleosomes, Gels, Geodetic position, Yeasts, Chromatin, Ribosomal DNA, Electrophoresis, Spheroplasts, Biochemistry
Were these topics helpful?See something inaccurate? Let us know!
Select the topics that are inaccurate.
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Preview not available
A simple no-background assay was developed for high-resolution in vivo analysis of yeast chromatin. When applied to Saccharomyces cerevisiae 5S rRNA genes (5S rDNA), this analysis shows that nucleosomes completely cover this chromosomal region, occupying alternative positions characterized by a unique helical phase. This supports the notion that sequence-intrinsic rotational signals are the major determinant of nucleosome localization. Nucleosomal core particles reconstituted in vitro occupy the same positions and have the same helically phased distribution observed in vivo, as determined by mapping of exonuclease III-resistant borders, mapping by restriction cleavages, and by DNase I and hydroxyl-radical digestion patterns.
Proceedings of the National Academy of Sciences of the United States of America © 1993 National Academy of Sciences