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Molecular Characterization of Monoclonal CRIA-Positive Anti-Arsonate Antibodies Derived from Idiotype-Negative Mice Bearing a Light Chain Polymorphism

Joel Tassignon, Maryse Brait, Ismaili Jamila, Jacques Urbain, Paul Gottlieb, Alan Brown, Charles A. Hasemann, J. Donald Capra and Katheryn Meek
Proceedings of the National Academy of Sciences of the United States of America
Vol. 90, No. 20 (Oct. 15, 1993), pp. 9508-9512
Stable URL: http://www.jstor.org/stable/2363184
Page Count: 5
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Molecular Characterization of Monoclonal CRIA-Positive Anti-Arsonate Antibodies Derived from Idiotype-Negative Mice Bearing a Light Chain Polymorphism
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Abstract

We have elicited anti-arsonate antibodies bearing the major cross-reactive idiotype (CRIA) in a double congenic idiotype-negative strain (C.C58.AL-20) bearing a light chain polymorphism that has previously been shown serologically not to complement idiotype-positive heavy chains. Using the idiotype cascade (Ab1 → Ab2 → Ab3 →→ Ab1'), CRIA-positive antibodies were raised and monoclonal antibodies were isolated and characterized serologically and by nucleotide sequence analysis. Two types of idiotype-positive anti-arsonate antibodies were generated in the C.C58.AL-20 strain. One group of hybridomas used the canonical VH1.8 heavy chain gene segment with Vκ10 variant light chains. A second group used a VHGAM3.8 heavy chain with Vκ10 variant light chains. This latter heavy-light pairing has been observed in CRIA-like responses previously in BALB/c mice after idiotypic manipulation (or rarely after antigen alone). These studies demonstrate the plasticity of the immune response when manipulated with idiotype reagents as well as its structural variability. Additionally, they provide important insights into the potentials of idiotype vaccines.

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