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Nuclear-Mitotic Apparatus Protein: A Structural Protein Interface Between the Nucleoskeleton and RNA Splicing
Changqing Zeng, Dacheng He, Susan M. Berget and B. R. Brinkley
Proceedings of the National Academy of Sciences of the United States of America
Vol. 91, No. 4 (Feb. 15, 1994), pp. 1505-1509
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/2364130
Page Count: 5
You can always find the topics here!Topics: Antibodies, Small nuclear ribonucleoproteins, Splicing, HeLa cells, RNA, Cells, Interphase, Monoclonal antibodies, Immunoprecipitation, Antigens
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Vertebrate splicing factors are localized to discrete domains within the nuclei of somatic cells. The mechanism whereby such nuclear domains, identified as speckles by immunofluorescence microscopy, are generated is unclear. Recent studies suggest that the spatial order within the nucleus is maintained by nuclear matrix factors. Here we show that a protein in the nuclear matrix and mitotic apparatus [nuclear-mitotic apparatus protein, NuMA; Lydersen, B. \& Pettijohn, D. (1980) Cell 22, 489-499] colocalizes with splicing factors in interphase nuclei and is associated with small nuclear ribonucleoproteins in a complex immunoprecipitated from HeLa extract with small nuclear ribonucleoprotein antibodies. Moreover, NuMA associates with splicing complexes that are reconstituted in vitro using wild-type pre-mRNA, but not with nonspecific RNA. Cumulatively, these observations suggest a function of NuMA or NuMA-like proteins in interphase cells in providing a bridge between RNA processing and the nucleoskeleton.
Proceedings of the National Academy of Sciences of the United States of America © 1994 National Academy of Sciences