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Use of Genetic Recombination as a Reporter of Gene Expression
Andrew Camilli, David T. Beattie and John J. Mekalanos
Proceedings of the National Academy of Sciences of the United States of America
Vol. 91, No. 7 (Mar. 29, 1994), pp. 2634-2638
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/2364289
Page Count: 5
You can always find the topics here!Topics: Bacteria, Gene expression, Infections, Journalism, Genes, Mice, Plasmids, Small intestine, Phosphatases, Aeration
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An understanding of the patterns of gene expression in response to specific environmental signals can yield insight into a variety of complex biological systems such as microbial-host interactions, developmental cycles, cellular differentiation, ontogeny, etc. To extend the utility of the reporter gene fusion approach to such studies, we have constructed a gene expression reporter cassette that permits the generation of transcriptional fusions to tnpR encoding resolvase, a site-specific recombinase of the transposable element γδ. Induction of the transcriptional fusions results in production of resolvase, which in turn, catalyzes excision of a linked tetracycline-resistance reporter gene flanked by direct repeats of res, the DNA sequences at which resolvase functions. The loss of tetracycline resistance in descendant bacteria serves as a permanent and heritable marker of prior gene expression. This gene fusion approach will allow us to assay the induction of gene expression in as few as one cell. Additionally, gene expression can be assayed at a later time and/or different place from the inducing environment facilitating the study of gene expression in complex environments such as animal tissues.
Proceedings of the National Academy of Sciences of the United States of America © 1994 National Academy of Sciences