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A Chloroplast Homologue of the Signal Recognition Particle Subunit SRP54 is Involved in the Posttranslational Integration of a Protein into Thylakoid Membranes

Xingxiang Li, Ralph Henry, Jianguo Yuan, Kenneth Cline and Neil E. Hoffman
Proceedings of the National Academy of Sciences of the United States of America
Vol. 92, No. 9 (Apr. 25, 1995), pp. 3789-3793
Stable URL: http://www.jstor.org/stable/2367432
Page Count: 5
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
A Chloroplast Homologue of the Signal Recognition Particle Subunit SRP54 is Involved in the Posttranslational Integration of a Protein into Thylakoid Membranes
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Abstract

The mechanisms involved in the integration of proteins into the thylakoid membrane are largely unknown. However, many of the steps of this process for the light-harvesting chlorophyll a/b protein (LHCP) have been described and reconstituted in vitro. LHCP is synthesized as a precursor in the cytosol and posttranslationally imported into chloroplasts. Upon translocation across the envelope membranes, the N-terminal transit peptide is cleaved, and the apoprotein is assembled into a soluble "transit complex" and then integrated into the thylakoid membrane via three transmembrane helices. Here we show that 54CP, a chloroplast homologue of the 54-kDa subunit of the mammalian signal recognition particle (SRP54), is essential for transit complex formation, is present in the complex, and is required for LHCP integration into the thylakoid membrane. Our data indicate that 54CP functions posttranslationally as a molecular chaperone and potentially pilots LHCP to the thylakoids. These results demonstrate that one of several pathways for protein routing to the thylakoids is homologous to the SRP pathway and point to a common evolutionary origin for the protein transport systems of the endoplasmic reticulum and the thylakoid membrane.

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