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The GroES Homolog of Helicobacter pylori Confers Protective Immunity Against Mucosal Infection in Mice
Richard L. Ferrero, Jean-Michel Thiberge, Imad Kansau, Nicole Wuscher, Michel Huerre and Agnes Labigne
Proceedings of the National Academy of Sciences of the United States of America
Vol. 92, No. 14 (Jul. 3, 1995), pp. 6499-6503
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/2367870
Page Count: 5
You can always find the topics here!Topics: Antigens, Infections, Bacteria, Antibodies, Mice, Dosage, Proteins, Gastritis, Immunity, Helicobacter
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Helicobacter pylori is an important etiologic agent of gastroduodenal disease. In common with other organisms, H. pylori bacteria express heat shock proteins that share homologies with the GroES-GroEL class of proteins from Escherichia coli. We have assessed the heat shock proteins of H. pylori as potential protective antigens in a murine model of gastric Helicobacter infection. Orogastric immunization of mice with recombinant H. pylori GroES- and GroEL-like proteins protected 80% (n = 20) and 70% (n = 10) of animals, respectively, from a challenge dose of 104 Helicobacter felis bacteria (compared to control mice, P = 0.0042 and P = 0.0904, respectively). All mice (n = 19) that were immunized with a dual antigen preparation, consisting of H. pylori GroES-like protein and the B subunit of H. pylori urease, were protected against infection. This represented a level of protection equivalent to that provided by a sonicated Helicobacter extract (P = 0.955). Antibodies directed against the recombinant H. pylori antigens were predominantly of the IgG1 class, suggesting that a type 2 T-helper cell response was involved in protection. This work reports a protein belonging to the GroES class of heat shock proteins that was shown to induce protective immunity. In conclusion, GroES-like and urease B-subunit proteins have been identified as potential components of a future H. pylori subunit vaccine.
Proceedings of the National Academy of Sciences of the United States of America © 1995 National Academy of Sciences