Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

FcεRI-Mediated Recruitment of p53/56lyn to Detergent- Resistant Membrane Domains Accompanies Cellular Signaling

Kenneth A. Field, David Holowka and Barbara Baird
Proceedings of the National Academy of Sciences of the United States of America
Vol. 92, No. 20 (Sep. 26, 1995), pp. 9201-9205
Stable URL: http://www.jstor.org/stable/2368438
Page Count: 5
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
FcεRI-Mediated Recruitment of p53/56lyn to Detergent- Resistant Membrane Domains Accompanies Cellular Signaling
Preview not available

Abstract

Detergent-resistant plasma membrane structures, such as caveolae, have been implicated in signaling, transport, and vesicle trafficking functions. Using sucrose gradient ultracentrifugation, we have isolated low-density, Triton X-100-insoluble membrane domains from RBL-2H3 mucosal mast cells that contain several markers common to caveolae, including a src-family tyrosine kinase, p53/56lyn. Aggregation of FcεRI, the high-affinity IgE receptor, causes a significant increase in the amount of p53/56lyn associated with these low-density membrane domains. Under our standard conditions for lysis, IgE-FcεRI fractionates with the majority of the solubilized proteins, whereas aggregated receptor complexes are found at a higher density in the gradient. Stimulated translocation of p53/56lyn is accompanied by increased tyrosine phosphorylation of several proteins in the low-density membrane domains as well as enhanced in vitro tyrosine kinase activity toward these proteins and an exogenous substrate. With a lower detergent-to-cell ratio during lysis, significant FcεRI remains associated with these membrane domains, consistent with the ability to coimmunoprecipitate tyrosine kinase activity with FcεRI under similar lysis conditions [Pribluda, V. S., Pribluda, C. \& Metzger, H. (1994) Proc. Natl. Acad. Sci. USA 91, 11246-11250]. These results indicate that specialized membrane domains may be directly involved in the coupling of receptor aggregation to the activation of signaling events.

Page Thumbnails

  • Thumbnail: Page 
9201
    9201
  • Thumbnail: Page 
9202
    9202
  • Thumbnail: Page 
9203
    9203
  • Thumbnail: Page 
9204
    9204
  • Thumbnail: Page 
9205
    9205