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Short-term Products of 14 C-acetate Assimilation by Chlorella pyrenoidosa in the Light

M. J. MERRETT and K. H. GOULDING
Journal of Experimental Botany
Vol. 18, No. 54 (February 1967), pp. 128-139
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/23686881
Page Count: 12
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Short-term Products of
          14
          C-acetate Assimilation by Chlorella pyrenoidosa in the Light
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Abstract

The kinetics of 14C-2-acetate assimilation by Chlorella pyrenoidosa in the light were examined. Under aerobic conditions the primary product of acetate assimilation was succinic acid which, after ten seconds, contained over 60 per cent of the 14C incorporated by the cells. The percentage of the total 14C in succinate fell with time, while that in citrate and glutamate increased. After 1800 sec over 60 per cent of 14C was present in two compounds, glutamic acid and an unknown compound (X). Glucose-6-phosphate, fructose-6-phosphate, phosphoglyceric acid and phosphoenolpyruvic acid became labelled after 60 sec but together never contained more than one per cent of the total 14C incorporated. Under anaerobic conditions succinate was still the primary product of acetate assimilation, and the absence of carbon dioxide resulted in a decrease in 14C incorporation into compound X. The pattern of acetate assimilation in acetate grown and acetate adapted Chlorella was very similar to that in photo-autotrophically grown Chlorella. In the presence of 10-6M DCMU, succinic acid was the primary product of acetate assimilation, but there was an early incorporation of 14C into glutamate, aspartate, and malate. 4 × 10-3M MFA did not effect the early incorporation of 14C into succinic acid, but resulted in accumulation of 14C in citrate and a decreased amount in glutamate and in compound X.

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