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Plastid Development in Primary Leaves of Phaseolus vulgaris: FREE NUCLEOTIDE CONCENTRATIONS IN GREENING LEAVES
M. E. WALLIS and J. W. BRADBEER
Journal of Experimental Botany
Vol. 21, No. 69 (November 1970), pp. 1039-1047
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/23687247
Page Count: 9
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The free nucleotides of 14-day-old dark-grown bean leaves have been extracted by perchloric acid and fractionated by ion-exchange chromatography. AMP, ATP, CMP, GMP, GDP, ADP-ribosephosphate (the acid-breakdown product of NADPH), NADP+, UMP, UDP-glucose, and UDP-xylose were the main components identified. During 45 h continuous illumination greening occurred and there was a 192 per cent increase in the total free nucleotide per leaf. Most of the constituent nucleotides showed rises during greening, the most notable increases being of substances concerned with photosynthesis, namely NADPH, and UDP-glucose. A rise in the NADPH/NADP+ ratio after 15 h illumination appeared to signify the onset of photosynthesis.
Journal of Experimental Botany © 1970 Oxford University Press