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Composition of Excised and Attached Sterile Pea Roots—Loss of Cell Nucleic Acids
A. J. ABBOTT
Journal of Experimental Botany
Vol. 23, No. 76 (August 1972), pp. 667-674
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/23687316
Page Count: 8
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The composition of excised cultured pea-root tips has been compared with aseptically cultured attached root tips. Expressed in cellular terms, the cultured excised roots showed a reduction in nitrogenous and nucleic acid constituents. The cells of attached roots had approximately 2-, 4-, 5- and 4-fold amounts of protein, soluble nitrogen, ribonucleic acid and deoxyribonucleic acid respectively, as compared with excised roots cultured in the same medium. Cultured root meristems did not contain the quiescent zone normally found in attached roots. It is suggested that the observed reduction in RNA and DNA per cell does not impair the ability of cells to divide and expand normally and, further, that there is surplus nucleic acid in attached root cells not obligatory for full expression of the genome.
Journal of Experimental Botany © 1972 Oxford University Press