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Changing Nucleosome Positions Through Modification of the DNA Rotational Information
Memmo Buttinelli, Rodolfo Negri, Lucia Di Marcotullio and Ernesto Di Mauro
Proceedings of the National Academy of Sciences of the United States of America
Vol. 92, No. 23 (Nov. 7, 1995), pp. 10747-10751
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/2368865
Page Count: 5
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The effects of the rotational information of DNA in determining the in vitro localization of nucleosomal core particles (ncps) have been studied in the Saccharomyces cerevisiae 5S rRNA repeat gene. We have altered the distribution of the phased series of flexibility signals present on this DNA by inserting a 25-bp tract, and we have analyzed the effects of this mutation on the distribution and on the frequencies of ncps, as compared with the wild type and a reference 21-bp insertion mutant. The variation of the standard free energy of nucleosome reconstitution was determined. The results show that the DNA rotational information is a major determinant of ncps positioning, define how many rotationally phased signals are required for the formation of a stable particle, and teach how to modify their distribution through the alteration of the rotational signals.
Proceedings of the National Academy of Sciences of the United States of America © 1995 National Academy of Sciences