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Effects of Four Detergents on the Oxidative and Phosphorylating Capacities of Potato Mitochondria
P. MAZLIAK and A. M. DECOTTE
Journal of Experimental Botany
Vol. 27, No. 99 (August 1976), pp. 769-777
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/23689132
Page Count: 9
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Oxidative and phosphorylating capacities of potato mitochondria were studied with an oxygen electrode; succinate was used as a respiratory substrate. When low concentrations (0.1 mg ml-1 or less) of four detergents (Triton X-100, Tween 80, sodium cholate, and sodium deoxycholate) were added into the medium, the respiratory rates, respiratory controls, and ADP/O ratios of mitochondria were increased. At higher concentrations, Triton X-100 and sodium deoxycholate were sharp inhibitors of the mitochondrial respiration; sodium cholate and over all Tween 80 were much less toxic for potato mitochondria.
Journal of Experimental Botany © 1976 Oxford University Press