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Ethylene Production and Plantlet Formation by Nicotiana Anthers Cultured in the Presence and Absence of Charcoal
M. HORNER, J. A. McCOMB, A. J. McCOMB and H. E. STREET
Journal of Experimental Botany
Vol. 28, No. 107 (December 1977), pp. 1365-1372
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/23689566
Page Count: 8
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Anthers of Nicotiana tabacum produce ethylene when cultured for plantlet production. The rate is at a maximum 1—2 weeks after the onset of culture. Charcoal in the medium increases the proportion of androgenic anthers in N. tabacum and several other Nicotiana species. The level of ethylene in culture vessels is reduced by charcoal. However, complete removal of ethylene does not significantly alter the incidence of androgenesis, nor does continuous flushing of cultures with air. It is concluded that although charcoal reduces ethylene in the gas phase of the cultures its effect on androgenesis is exerted through some other mechanism.
Journal of Experimental Botany © 1977 Oxford University Press