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Isolation, Culture, and Plant Regeneration of Protoplasts of Two Shrubby Oxalis Species from South America

S. J. OCHATT, A. S. ESCANDON and A. J. MARTINEZ
Journal of Experimental Botany
Vol. 40, No. 213 (April 1989), pp. 493-496
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/23692246
Page Count: 4
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Isolation, Culture, and Plant Regeneration of Protoplasts of Two Shrubby Oxalis Species from South America
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Abstract

Protoplasts were successfully isolated from internodal callus tissues of both Oxalis glaucifolia and O. rhombeo-ovata when they were digested in a solution containing 0.1% (w/v) Macerozyme R-10, 0.5% (w/v) cellulase Onozuka R-10 and 0.3 mmol m-3 sucrose. Protoplasts proliferated to give cell colonies on Gamborg et al.'s B5 medium supplemented with 0.3 mmol m-3 mannitol, 0.5 mg dm-3 2,4-D, and 2.0 mg dm-3 kinetin. Callus was produced upon transfer of cell colonies to Murashige and Skoog medium containing 2.0 mg dm-3 1-naphthaleneacetic acid (NAA) and 0.1 mg dm-3 kinetin for O. glaucifolia, or with 5.0 mg dm-3 NAA and 0.5 mg dm-3 6-benzylaminopurine, for O. rhombeo-ovata. Plants were regenerated from O. glaucifolia protoplasts on a medium containing 0.1 mg dm-3 NAA, 1.0 mg dm-3 kinetin and 1.0 mg dm-3 gibberellic acid, but only vascular nodules were differentiated by O. rhombeo-ovata protoplast-derived calli.

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