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Permeability of Isolated Infected Cells from Soybean Nodules

YOUZHONG LI and DAVID A. DAY
Journal of Experimental Botany
Vol. 42, No. 243 (October 1991), pp. 1325-1329
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/23693768
Page Count: 5
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Permeability of Isolated Infected Cells from Soybean Nodules
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Abstract

Infected cells from the central zone of mature soybean nodules were isolated by incubating nodule slices with hydrolytic enzymes, and their permeability to various organic compounds measured. The cells were effectively impermeable to sucrose, fructose and glucose, readily permeable to malate and succinate and partially permeable to glutamate and glutamine. Malate uptake showed saturation kinetics and was competitively inhibited by succinate. Malate uptake was also inhibited by a protonophore and the respiratory poison antimycin, as well as by butylmalonate, cyanocinnamic acid and other substrate analogues, but not by phthalonate. We conclude that there is a dicarboxylate carrier on the plasmamembrane of soybean infected cells, which catalyses the uptake of malate and which depends on mitochondrial ATP for maximum rates.

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