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Nickel and Cadmium-related Changes in Growth, Plasma Membrane Lipid Composition, ATPase Hydrolytic Activity and Proton-pumping of Rice (Oryza sativa L. cv. Bahía) Shoots

ROC ROS, DAVID T. COOKE, CARMEN MARTINEZ-CORTINA and ISABEL PICAZO
Journal of Experimental Botany
Vol. 43, No. 256 (November 1992), pp. 1475-1481
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/23694276
Page Count: 7
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Nickel and Cadmium-related Changes in Growth, Plasma Membrane Lipid Composition, ATPase Hydrolytic Activity and Proton-pumping of Rice (Oryza sativa L. cv. Bahía) Shoots
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Abstract

Six-day-old rice plants (Oryza sativa L. cv. Bahía) were grown in the presence of 0.5 mol m-3 Ni or Cd for 5 or 10 d. Five days after treatment, some plants were transferred to a medium containing no heavy metal for 5 or 10 d. Plasmalemma vesicles from shoots of treated, transferred (recovery experiments) and control plants were isolated. ATPase activity and proton-pumping were assessed, and free sterols and phospholipids determined. The ATPase hydrolytic activity was increased by 37% and 34% in 5 and 10 d Cd-treated plants, respectively; and by 66% in 5 and 10 d Ni-treated plants. However, neither the initial rate of H+ transport nor the proton-pumping rate at steady-state were significantly affected by the treatments. The relative proportion (%) of the plasmalemma sterols campesterol and Δ5-avenasterol decreased while sitosterol increased during heavy metal treatment. The overall plasmalemma phospholipid fatty acyl chain length and degree of unsaturation were also reduced. In experiments where plants recovered from Cd and Ni treatment, differences between treated and control plants were reverted, particularly in 10 d Ni-recovered plants. The possible involvement of lipids in the regulation of the plasmalemma ATPase as well as the relationship between growth, ATPase and adaptations to stress conditions are discussed.

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