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Regulation of IAA—Amino Acid Conjugate Formation in Carrot Tissues Transformed by Agrobacterium tumefaciens

KAZUO SASAKI, SHINGO SAKAI, HIROSHI KAMADA and HIROSHI HARADA
Journal of Experimental Botany
Vol. 43, No. 256 (November 1992), pp. 1491-1495
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/23694279
Page Count: 5
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Regulation of IAA—Amino Acid Conjugate Formation in Carrot Tissues Transformed by Agrobacterium tumefaciens
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Abstract

Regulation of IAA—amino acid conjugate formation was examined in cultured carrot tissues transformed with Agrobacterium tumefaciens harbouring aux- Ti plasmids. IAA—conjugate formation in carrot tissues transformed with aux- Ti plasmids was induced by exogenously-applied IAA. When these tissues were treated with an inhibitor of protein synthesis (cycloheximide) before IAA treatment, the induction of IAA—conjugate formation was completely inhibited. By contrast, pre-treatment with an inhibitor of RNA synthesis (actinomycin D) before IAA treatment reduced induction of IAA—conjugate formation. These results suggest that IAA-inducible IAA—conjugate is catabolized by enzyme(s) and the formation of conjugated IAA is regulated at the transcriptional level in carrot. Presumably, in carrot tissues transformed with wild-type or cyt- Ti plasmids, the same mechanism(s) exists and the free IAA content in the carrot tissues is maintained at a low level.

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