You are not currently logged in.
Access JSTOR through your library or other institution:
Expression of the gus A gene in embryogenic cell lines of Sitka spruce following Agrobacterium-mediated transformation
P.M.W. Drake, A. John, J.B. Power and M.R. Davey
Journal of Experimental Botany
Vol. 48, No. 306 (JANUARY 1997), pp. 151-155
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/23695567
Page Count: 5
You can always find the topics here!Topics: Agrobacterium, Inoculation, Conifers, Cell lines, Gene expression, Plant cells, Chemical suspensions, Plants, Transformed cell line, Somatic embryogenesis
Were these topics helpful?See somethings inaccurate? Let us know!
Select the topics that are inaccurate.
Preview not available
Transformation of Picea sitchensis (Bong) Carr. was investigated by incubating embryogenic cell lines, initiated from immature and mature zygotic embryos, with a supervirulent strain of Agrobacterium tumefaciens. The latter carried a gus A-intron gene. Transient gene expression was determined histochemically by recording the number of distinct areas of β-glucuronidase (GUS) activity. Maximum expression of the gus gene was achieved with a bacterial suspension with an OD600 of 0.8-1.1 diluted with an equal volume of MPM medium, inoculation of cells with bacteria for 30 min, 72 h co-cultivation period and exposure of Agrobacterium and plant cells to 50 μM acetosyringone. These results are discussed in relation to Agrobacterium-mediated gene delivery for the stable transformation of Sitka spruce and other conifers.
Journal of Experimental Botany © 1997 Oxford University Press