Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Identification of whitefish remains by mtDNA analysis

Paweł Brzuzan, Sławomir Ciesielski, Jan Kusznierz and Łukasz Jurczyk
Annales Zoologici Fennici
Vol. 41, No. 1, Biology and Management of Coregonid Fishes: Proceedings of the Eighth International Symposium on the Biology and Management of Coregonid Fishes, Rovaniemi, Finland, 26—29 August 2002 (2004), pp. 61-68
Stable URL: http://www.jstor.org/stable/23736187
Page Count: 8
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Identification of whitefish remains by mtDNA analysis
Preview not available

Abstract

Remains of Coregonus sp. were obtained from the collection of archeological materials found in 1991 at the site Wola Grzymalina, Poland. DNA has been extracted from vertebrae that were dated about 500 000 years old. Polymerase chain reactions on extracts from the vertebral remains were succesful only using primers that targeted nucleotide sequences that were not longer than about 100bp. The ancient nucleotide sequence of a 102bp fragment of mitochondrial DNA ND1 gene matched the contemporary sequence of European whitefish (Coregonus lavaretus) and differed from that of vendace (C. albula) by four substitutions. Ambiguous nucleotides were observed at nine positions throughout the 87bp fragment of mtDNA control region (CR), nonetheless the sequence was different from that of vendace by eight additional changes. Phylogenetic analysis utilizing Bayesian inference placed the archival sequence into a clade with European whitefish. The support for this reconstruction was f(τ|X) = 0.65 after analysis of the ND1 fragment and f(τ|X) = 0.52 after analysis of the CR fragment. Excellent preservation of the specimen analysed in this study proves the potential for analysing genetic characteristics of species and specimens that are thousands of years old.

Page Thumbnails

  • Thumbnail: Page 
[61]
    [61]
  • Thumbnail: Page 
62
    62
  • Thumbnail: Page 
63
    63
  • Thumbnail: Page 
64
    64
  • Thumbnail: Page 
65
    65
  • Thumbnail: Page 
66
    66
  • Thumbnail: Page 
67
    67
  • Thumbnail: Page 
68
    68