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Identification of whitefish remains by mtDNA analysis
Paweł Brzuzan, Sławomir Ciesielski, Jan Kusznierz and Łukasz Jurczyk
Annales Zoologici Fennici
Vol. 41, No. 1, Biology and Management of Coregonid Fishes: Proceedings of the Eighth International Symposium on the Biology and Management of Coregonid Fishes, Rovaniemi, Finland, 26—29 August 2002 (2004), pp. 61-68
Published by: Finnish Zoological and Botanical Publishing Board
Stable URL: http://www.jstor.org/stable/23736187
Page Count: 8
You can always find the topics here!Topics: DNA, Mitochondrial DNA, Polymerase chain reaction, Whitefish, Bones, Lakes, Phylogenetics, Sequencing, Evolution, Nucleotide sequences
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Remains of Coregonus sp. were obtained from the collection of archeological materials found in 1991 at the site Wola Grzymalina, Poland. DNA has been extracted from vertebrae that were dated about 500 000 years old. Polymerase chain reactions on extracts from the vertebral remains were succesful only using primers that targeted nucleotide sequences that were not longer than about 100bp. The ancient nucleotide sequence of a 102bp fragment of mitochondrial DNA ND1 gene matched the contemporary sequence of European whitefish (Coregonus lavaretus) and differed from that of vendace (C. albula) by four substitutions. Ambiguous nucleotides were observed at nine positions throughout the 87bp fragment of mtDNA control region (CR), nonetheless the sequence was different from that of vendace by eight additional changes. Phylogenetic analysis utilizing Bayesian inference placed the archival sequence into a clade with European whitefish. The support for this reconstruction was f(τ|X) = 0.65 after analysis of the ND1 fragment and f(τ|X) = 0.52 after analysis of the CR fragment. Excellent preservation of the specimen analysed in this study proves the potential for analysing genetic characteristics of species and specimens that are thousands of years old.
Annales Zoologici Fennici © 2004 Finnish Zoological and Botanical Publishing Board