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A novel method for sex determination of giant panda
Xiao Xu, Kun Wei, Wen-Ping Zhang, Ling Lin, Xiao-Fang Wang, Fu-Jun Shen, Liang Zhang, Zhi-He Zhang and Bi-Song Yue
Annales Zoologici Fennici
Vol. 44, No. 6 (2007), pp. 401-404
Published by: Finnish Zoological and Botanical Publishing Board
Stable URL: http://www.jstor.org/stable/23736841
Page Count: 4
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A fast, inexpensive, and reliable method for sex determination of giant panda was developed using amplification of giant panda zinc finger alleles (ZFX/ZFY). Two sets of primers targeted a conserved region in the exon of the ZFX/ZFY genes worked in a single PCR process, and provided positive results for both males and females. Sexes of individuals were revealed by sex-specific banding patterns (females: 447 bp, 146 bp; males: 447 bp, 350 bp, 146 bp). This method was successfully tested on blood sample from pandas of known sex. Non-invasive tests revealed that this method can also be applied to non-invasive samples such as hair or feces.
Annales Zoologici Fennici © 2007 Finnish Zoological and Botanical Publishing Board